Application
Western blot analysis on (A) HeLa + Camptothecin, (B) HeLa, (C) Jurkat + Staurosporine and (D) Jurkat cell lysates using anti-Cleaved alpha-Fodrin (Asp1185) RabMAb (Cat. #AJ1191a).| Product Name | Cleaved alpha-Fodrin Antibody (Asp1185) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR3830 |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:10000~50000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | NEAS SPTA2 |
|---|---|
| Molecular Weight(MW) | 284539 Da |
| Function | Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane |
| Tissue Specificity | A synthetic peptide corresponding to residues in human Alpha Fodrin was used as an immunogen. This antibody only detects the large fragment of Alpha Fodrin resulting from cleavage at aspartic acid 1185. |
| Cellular Localization | Cytoplasm, cytoskeleton. Cytoplasm, cell cortex. Note=Expressed along the cell membrane in podocytes and presumptive tubule cells during glomerulogenesis and is expressed along lateral cell margins in tubule cells (By similarity) |
| Entrez Gene | 6709 |
|---|
Application
Western blot analysis on (A) HeLa + Camptothecin, (B) HeLa, (C) Jurkat + Staurosporine and (D) Jurkat cell lysates using anti-Cleaved alpha-Fodrin (Asp1185) RabMAb (Cat. #AJ1191a).| Application Notes | WB:1:10000~50000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
