Immunoblot
1: 50ug HEK lysatePrimary Antibody Dilution: 1:100Secondary Antibody: Anti-rabbit-RPFSecondary Antibody Dilution: 1:10,000Gene Name: BECN1Submitted by: Agathe Korniat INSERM/UPMC UMRS_956Western blot
0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: 721_B cell lysate| Product Name | Rabbit anti-BECN1 polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ATG6, VPS30, beclin1 |
| Immunogen | The immunogen for anti-BECN1 antibody: synthetic peptide directed towards the middle region of human BECN1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogGuinea PigHumanMousePigRabbitRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BECN1 |
|---|---|
| Gene Synonyms | ATG6 VPS30 beclin1 |
| Gene Full Name | Beclin 1, autophagy related |
| Gene Summary | BECN1 plays a central role in autophagy. BECN1 may play a role in antiviral host defense. BECN1 protects against infection by a neurovirulent strain of Sindbis virus. |
| Alternative Names | ATG6 VPS30 beclin1 |
| Molecular Weight(MW) | 50kDa |
| Sequence | 450 amino acids |
Immunoblot
1: 50ug HEK lysatePrimary Antibody Dilution: 1:100Secondary Antibody: Anti-rabbit-RPFSecondary Antibody Dilution: 1:10,000Gene Name: BECN1Submitted by: Agathe Korniat INSERM/UPMC UMRS_956
Western blot
0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: 721_B cell lysate| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
