Western blot
500 ug mouse brain homogenateAmount of IP Antibody :6 ugPrimary Antibody :PSEN1Primary Antibody Dilution :1:500Secondary Antibody :Goat anti-rabbit Alexa-Fluor 594Secondary Antibody Dilution :1:5000Gene Name :PSEN1Submitted by :Dr. Yuzhi Chen, University of Arkansas for Medical ScienceImmunohistochemical
The PSEN1 antibody (# 58941) works fine in 4% paraformaldehyde fixed tissue sections (30 um), they were cut on a sliding, freezing microtome. We stained it overnight 1:500 followed with a biotinylated secondary, followed by an Avidin/peroxidase tertiary antibody, final staining with metal-intensified DAB. Similarly, both LRP antibodies (#58562 & 58563) work fine (stain appropriately) with this procedure. We are still evaluating the BACE1 antibody, it seems to work, too. -Dr. Thomas van GroenImmunohistochemistry
Immunohistochemistry with Small intestine tissueImmunoblot
1. Mouse WT brain extract (80ug) 2. Rat brain extract (80ug)Primary Antibody Dilution: 2ug/mlSecondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR BioscienceSecondary Antibody Dilution: 1: 20,000Gene Name: PSEN1Submitted by: Dr. Yuzhi Chen, University of Arkansas for Medical ScienceWestern blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Human brain| Product Name | Rabbit anti-PSEN1 polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AD3, FAD, PS1, S182, PS-1 |
| Immunogen | The immunogen for anti-PSEN1 antibody: synthetic peptide directed towards the middle region of human PSEN1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHCIP |
| WB:1:500~1:2000 IHC:1:50~1:200 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineGuinea PigHorseHumanMousePig |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PSEN1 |
|---|---|
| Gene Synonyms | AD3 FAD PS1 S182 PS-1 |
| Gene Full Name | Presenilin 1 |
| Gene Summary | Alzheimer's disease (AD) patients with an inherited form of the disease carry mutations in the presenilin proteins (PSEN1; PSEN2) or in the amyloid precursor protein (APP). These disease-linked mutations result in increased production of the longer form of amyloid-beta (main component of amyloid deposits found in AD brains). Presenilins are postulated to regulate APP processing through their effects on gamma-secretase, an enzyme that cleaves APP. Also, it is thought that the presenilins are involved in the cleavage of the Notch receptor, such that they either directly regulate gamma-secretase activity or themselves are protease enzymes. Several alternatively spliced transcript variants encoding different isoforms have been identified for this gene, the full-length nature of only some have been determined. |
| Alternative Names | AD3 FAD PS1 S182 PS-1 |
| Molecular Weight(MW) | 53kDa |
| Sequence | 467 amino acids |
Western blot
500 ug mouse brain homogenateAmount of IP Antibody :6 ugPrimary Antibody :PSEN1Primary Antibody Dilution :1:500Secondary Antibody :Goat anti-rabbit Alexa-Fluor 594Secondary Antibody Dilution :1:5000Gene Name :PSEN1Submitted by :Dr. Yuzhi Chen, University of Arkansas for Medical Science
Immunohistochemical
The PSEN1 antibody (# 58941) works fine in 4% paraformaldehyde fixed tissue sections (30 um), they were cut on a sliding, freezing microtome. We stained it overnight 1:500 followed with a biotinylated secondary, followed by an Avidin/peroxidase tertiary antibody, final staining with metal-intensified DAB. Similarly, both LRP antibodies (#58562 & 58563) work fine (stain appropriately) with this procedure. We are still evaluating the BACE1 antibody, it seems to work, too. -Dr. Thomas van Groen
Immunohistochemistry
Immunohistochemistry with Small intestine tissue
Immunoblot
1. Mouse WT brain extract (80ug) 2. Rat brain extract (80ug)Primary Antibody Dilution: 2ug/mlSecondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR BioscienceSecondary Antibody Dilution: 1: 20,000Gene Name: PSEN1Submitted by: Dr. Yuzhi Chen, University of Arkansas for Medical Science
Western blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Human brain| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
