Application
Immunohistochemistry of Presenilin1 in human brain tissue with Presenilin1 antibody at 2.5 μg/mL.Application
Immunofluorescence of Presenilin1 in Human Brain cells with Presenilin1 antibody at 20 μg/mL.Application
Western blot analysis of presenilin1 in human brain lysate with presenilin1 antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.| Product Name | Presenilin1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Presenilin1 Antibody: AD3, FAD, PS1, PS-1, S182, AD3, PSNL1, Presenilin-1, Protein S182 |
| Product description | Presenilin1 Antibody: Presenilin1 was initially identified a marker of susceptibility to early-onset Alzheimer's disease. In addition to PEN2, nicastrin and APH-1, Presenilin1 forms the gamma-secretase protein complex, a membrane-bound aspartyl protease that can cleave certain proteins at peptide bonds buried within the hydrophobic environment of the lipid bilayer. This cleavage is responsible for a key step in signaling from several cell-surface receptors and is thought to be required for the generation of the neurotoxic amyloid peptides that are central to the pathogenesis of Alzheimer's disease. Like the tumor necrosis factor-alpha-converting enzyme (TACE) and the beta-site cleavage enzyme (BACE) protease families, gamma-secretase will cleave the amyloid precursor protein (APP), but within the intramembrane region of APP, resulting in either the non-toxic p3 (from the alpha and gamma cleavage site) or the toxic Abeta amyloid peptide (from the beta and gamma cleavage site). It is thought that accumulation of the Abeta peptide is the precursor to Alzheimer's disease. Multiple isoforms of presenilin1 are known to exist. This antibody has no cross-reactivity to presenilin2.1) Sherrington R, Rogaev EI, Liang Y, et al. Cloning of a gene bearing missense mutations in early-onset familial Alzheimer’s disease. Nature1995; 375:754-60. |
| Immunogen | Presenilin1 antibody was raised against a 23 amino acid synthetic peptide from near the carboxy terminus of human presenilin1. The immunogen is located within amino acids 330 - 380 of Presenilin1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| Presenilin1 antibody can be used for detection of presenilin1 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PSEN1 |
|---|---|
| Alternative Names | Presenilin1 Antibody: AD3 FAD PS1 PS-1 S182 AD3 PSNL1 Presenilin-1 Protein S182 |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
Application
Immunohistochemistry of Presenilin1 in human brain tissue with Presenilin1 antibody at 2.5 μg/mL.
Application
Immunofluorescence of Presenilin1 in Human Brain cells with Presenilin1 antibody at 20 μg/mL.
Application
Western blot analysis of presenilin1 in human brain lysate with presenilin1 antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.| Positive Control | 1) Cat. No. 1303 - Human Brain Tissue Lysate |
|---|---|
| Application Notes | Presenilin1 antibody can be used for detection of presenilin1 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Presenilin1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Presenilin1 Antibody is supplied in PBS containing 0.02% sodium azide. |
