Western blot
Host: Rabbit Target Name: Nae1 Sample Tissue: Mouse Lung lysates Antibody Dilution: 1.0ug/ml| Product Name | Rabbit anti-Nae1 Antibody - C-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 59kDa, Appbp1 |
| Immunogen | The immunogen for Anti-Nae1 antibody is: synthetic peptide directed towards the C-terminal region of Mouse Nae1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | MouseHuman |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Nae1 |
|---|---|
| Gene Synonyms | 59kDa Appbp1 |
| Gene Full Name | NEDD8 activating enzyme E1 subunit 1 |
| Gene Summary | Nae1 is a regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation. |
| Alternative Names | 59kDa Appbp1 |
| Molecular Weight(MW) | 60kDa |
| Sequence | 534 amino acids |
Western blot
Host: Rabbit Target Name: Nae1 Sample Tissue: Mouse Lung lysates Antibody Dilution: 1.0ug/ml| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
