Immunoblot
1: 10ug proteasome fraction from C57B1/6J mouse brain, 2: 10ug proteasome fraction from BLAB/C mouse brainPrimary Antibody Dilution: 1:500Secondary Antibody: Anti-rabbit HRPSecondary Antibody Dilution: 1:5000Gene Name: PSMC6Submitted by: Dr. Scott Wilson, University of Alabama at BirminghamWestern blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Jurkat cell lysate| Product Name | Rabbit anti-PSMC6 polyclonal antibody - C-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CADP44, MGC12520, P44, SUG2, p42 |
| Immunogen | The immunogen for anti-PSMC6 antibody: synthetic peptide directed towards the C terminal of human PSMC6 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | DogHorseMousePigRabbitRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PSMC6 |
|---|---|
| Gene Synonyms | CADP44 MGC12520 P44 SUG2 p42 |
| Gene Full Name | Proteasome (prosome, macropain) 26S subunit, ATPase, 6 |
| Gene Summary | The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases which have a chaperone-like activity. Pseudogenes have been identified on chromosomes 8 and 12. |
| Alternative Names | CADP44 MGC12520 P44 SUG2 p42 |
| Molecular Weight(MW) | 44kDa |
| Sequence | 389 amino acids |
Immunoblot
1: 10ug proteasome fraction from C57B1/6J mouse brain, 2: 10ug proteasome fraction from BLAB/C mouse brainPrimary Antibody Dilution: 1:500Secondary Antibody: Anti-rabbit HRPSecondary Antibody Dilution: 1:5000Gene Name: PSMC6Submitted by: Dr. Scott Wilson, University of Alabama at Birmingham
Western blot
0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Jurkat cell lysate| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
