Western blot
Sample Type: 1. Hamster CHO K1 cells (20ug)2. HeLa skin cells(100ug)3. HEK273 cells (100ug)4. HepG2 cells (100ug)5. purified human ADSL protein (40ng)Primary Dilution: 1:2000Secondary Antibody: Clean-Blot IP detection Reagent and KitSecondary Dilution: 1:2000Image Submitted By: V. Skopova, M. ZikanovaInstitute of Inherited Metabolic Disorders, Charles University in Prague and General Hopspital in PragueWestern blot
0.2-1 ug/mlELISA Titer: 1:62500Positive Control: Human Muscle| Product Name | Rabbit anti-ADSL polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AMPS, ASASE, ASL |
| Immunogen | The immunogen for anti-ADSL antibody: synthetic peptide directed towards the middle region of human ADSL |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogGuinea PigHorseHumanMousePigRabbitRatSheep |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ADSL |
|---|---|
| Gene Synonyms | AMPS ASASE ASL |
| Gene Full Name | Adenylosuccinate lyase |
| Gene Summary | Adenylsuccinate lyase is involved in both de novo synthesis of purines and formation of adenosine monophosphate from inosine monophosphate. It catalyzes two reactions in AMP biosynthesis: the removal of a fumarate from succinylaminoimidazole carboxamide (SAICA) ribotide to give aminoimidazole carboxamide ribotide (AICA) and removal of fumarate from adenylosuccinate to give AMP. Adenylosuccinase deficiency results in succinylpurinemic autism, psychomotor retardation, and , in some cases, growth retardation associated with muscle wasting and epilepsy. Two transcript variants encoding different isoforms have been found for this gene. |
| Alternative Names | AMPS ASASE ASL |
| Molecular Weight(MW) | 55kDa |
| Sequence | 484 amino acids |
Western blot
Sample Type: 1. Hamster CHO K1 cells (20ug)2. HeLa skin cells(100ug)3. HEK273 cells (100ug)4. HepG2 cells (100ug)5. purified human ADSL protein (40ng)Primary Dilution: 1:2000Secondary Antibody: Clean-Blot IP detection Reagent and KitSecondary Dilution: 1:2000Image Submitted By: V. Skopova, M. ZikanovaInstitute of Inherited Metabolic Disorders, Charles University in Prague and General Hopspital in Prague
Western blot
0.2-1 ug/mlELISA Titer: 1:62500Positive Control: Human Muscle| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
