Immunohistochemical
Sample Type :Monkey adrenal gland Primary Antibody Dilution : 1:25 Secondary Antibody:Anti-rabbit-HRP Secondary Antibody Dilution: 1:1000 Color/Signal Descriptions:Brown: CYP11B1 Blue: Nucleus Gene Name:CYP11B1 Submitted by:Jonathan Bertin, Endoceutics Inc.Immunohistochemical
Sample Type :Monkey vagina Primary Antibody Dilution : 1:25 Secondary Antibody:Anti-rabbit-HRP Secondary Antibody Dilution: 1:1000 Color/Signal Descriptions:Brown: CYP11B1 Blue: Nucleus Gene Name:CYP11B1 Submitted by:Jonathan Bertin, Endoceutics Inc.Western blot
Sample type: 1. HEK293TN-GFP-hcyp11B1 (75ug)2. HEK293TN-GFP-hcyp11B2 (75ug)Primary dilution: 1:100Secondary Antibody: mouse anti-Rabbit HRPSecondary Dilution: 1:10,000Film Exposed for: 5 minutesImage Submitted by: Celso Gomez-SanchezMongomery VA Medical CenteWestern blot
0.2-1 ug/mlELISA Titer: 1:62500Positive Control: Human Live| Product Name | Rabbit anti-CYP11B1 polyclonal antibody - C-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CPN1, CYP11B, DKFZp686B05283, FHI, FLJ36771, P450C11 |
| Immunogen | The immunogen for anti-CYP11B1 antibody: synthetic peptide directed towards the C terminal of human CYP11B1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CYP11B1 |
|---|---|
| Gene Synonyms | CPN1 CYP11B DKFZp686B05283 FHI FLJ36771 P450C11 |
| Gene Full Name | Cytochrome P450, family 11, subfamily B, polypeptide 1 |
| Gene Summary | This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the mitochondrial inner membrane and is involved in the conversion of progesterone to cortisol in the adrenal cortex. Mutations in this gene cause congenital adrenal hyperplasia due to 11-beta-hydroxylase deficiency. Transcript variants encoding different isoforms have been noted for this gene. |
| Alternative Names | CPN1 CYP11B DKFZp686B05283 FHI FLJ36771 P450C11 |
| Molecular Weight(MW) | 55kDa |
| Sequence | 503 amino acids |
Immunohistochemical
Sample Type :Monkey adrenal gland Primary Antibody Dilution : 1:25 Secondary Antibody:Anti-rabbit-HRP Secondary Antibody Dilution: 1:1000 Color/Signal Descriptions:Brown: CYP11B1 Blue: Nucleus Gene Name:CYP11B1 Submitted by:Jonathan Bertin, Endoceutics Inc.
Immunohistochemical
Sample Type :Monkey vagina Primary Antibody Dilution : 1:25 Secondary Antibody:Anti-rabbit-HRP Secondary Antibody Dilution: 1:1000 Color/Signal Descriptions:Brown: CYP11B1 Blue: Nucleus Gene Name:CYP11B1 Submitted by:Jonathan Bertin, Endoceutics Inc.
Western blot
Sample type: 1. HEK293TN-GFP-hcyp11B1 (75ug)2. HEK293TN-GFP-hcyp11B2 (75ug)Primary dilution: 1:100Secondary Antibody: mouse anti-Rabbit HRPSecondary Dilution: 1:10,000Film Exposed for: 5 minutesImage Submitted by: Celso Gomez-SanchezMongomery VA Medical Cente
Western blot
0.2-1 ug/mlELISA Titer: 1:62500Positive Control: Human Live| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
