Western blot
500 ug mouse brain homogenateAmount of IP Antibody :6 ugPrimary Antibody :TUBA1CPrimary Antibody Dilution :1:500Secondary Antibody :Goat anti-rabbit Alexa-Fluor 594Secondary Antibody Dilution :1:5000Gene Name :TUBA1CSubmitted by :Dr. Yuzhi Chen, University of Arkansas for Medical ScienceImmunohistochemical
Human brain stem cellsPrimary Antibody Dilution :1:500Secondary Antibody :Goat anti-rabbit Alexa-Fluor 594Secondary Antibody Dilution :1:1000Color/Signal Descriptions :TUBA1C: Red DAPI:BlueGene Name :TUBA1CSubmitted by :Dr. Yuzhi Chen, University of Arkansas for Medical ScienceWestern blot
Sample Type: 1. Human NT-2 cells (60ug) 2. and 3. mouse brain extracts (80ug) 4. rat brain extract (80ug) Primary Antibody Dilution: 2ug/ml Secondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR Bioscience Secondary Antibody Dilution: 1: 20,000 Image Submitted by: Yuzhi Chen University of Arkansas for Medical ScienceÂWestern blot
TUBA1C antibody - C-terminal region validated by WB using Placenta Lysate at 1.0ug/ml.| Product Name | Rabbit anti-TUBA1C polyclonal antibody - C-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MGC10851, MGC14580, TUBA6, bcm948 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHCIP |
| WB:1:500~1:2000 IHC:1:50~1:200 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TUBA1C |
|---|---|
| Gene Synonyms | MGC10851 MGC14580 TUBA6 bcm948 |
| Gene Full Name | Tubulin, alpha 1c |
| Gene Summary | Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. |
| Alternative Names | MGC10851 MGC14580 TUBA6 bcm948 |
| Molecular Weight(MW) | 50kDa |
| Sequence | 449 amino acids |
Western blot
500 ug mouse brain homogenateAmount of IP Antibody :6 ugPrimary Antibody :TUBA1CPrimary Antibody Dilution :1:500Secondary Antibody :Goat anti-rabbit Alexa-Fluor 594Secondary Antibody Dilution :1:5000Gene Name :TUBA1CSubmitted by :Dr. Yuzhi Chen, University of Arkansas for Medical Science
Immunohistochemical
Human brain stem cellsPrimary Antibody Dilution :1:500Secondary Antibody :Goat anti-rabbit Alexa-Fluor 594Secondary Antibody Dilution :1:1000Color/Signal Descriptions :TUBA1C: Red DAPI:BlueGene Name :TUBA1CSubmitted by :Dr. Yuzhi Chen, University of Arkansas for Medical Science
Western blot
Sample Type: 1. Human NT-2 cells (60ug) 2. and 3. mouse brain extracts (80ug) 4. rat brain extract (80ug) Primary Antibody Dilution: 2ug/ml Secondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR Bioscience Secondary Antibody Dilution: 1: 20,000 Image Submitted by: Yuzhi Chen University of Arkansas for Medical ScienceÂ
Western blot
TUBA1C antibody - C-terminal region validated by WB using Placenta Lysate at 1.0ug/ml.| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
