Application
Flow cytometric analysis of MCF-7 cells using TUBA1C Antibody (N-term)(green) compared to an isotype control of rabbit IgG(blue). Antibody was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.Application
Western blot analysis of lysates from HT-1080, T47D cell line, mouse brain and testis tissue lysate (from left to right), using TUBA1C Antibody at 1:1000 at each lane.| Product Name | TUBA1C Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Tubulin alpha-1C chain, Alpha-tubulin 6, Tubulin alpha-6 chain, TUBA1C, TUBA6 |
| Product description | Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.1) Lubec G.,et al.Submitted (MAR-2007) to UniProtKB. |
| Immunogen | This TUBA1C antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 26-60 amino acids from the N-terminal region of human TUBA1C. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For FACS starting dilution is: 1:25 For WB starting dilution is: 1:1000: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TUBA1C |
|---|---|
| Alternative Names | Tubulin alpha-1C chain Alpha-tubulin 6 Tubulin alpha-6 chain TUBA1C TUBA6 |
| Molecular Weight(MW) | 50 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Hamster |
Application
Flow cytometric analysis of MCF-7 cells using TUBA1C Antibody (N-term)(green) compared to an isotype control of rabbit IgG(blue). Antibody was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
Application
Western blot analysis of lysates from HT-1080, T47D cell line, mouse brain and testis tissue lysate (from left to right), using TUBA1C Antibody at 1:1000 at each lane.| Application Notes | For FACS starting dilution is: 1:25 For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
