Immunoblot
Lane 1: 20ug A549 cell lysateLane 2: 20ug H1437 cell lysateLane 3: 20ug PC3 cell lysatePrimary Antibody Dilution: 1:500Secondary Antibody: Anti-rabbit-HRPSecondary Antibody Dilution: 1:1000Gene Name: EFNA4Submitted by: Erika Mathes Lisabeth, Sanford-Burnham Medical Research InstituteWestern blot
Titration: 1.0 ug/mlPositive Control: MCF7 Whole Cell| Product Name | Rabbit anti-EFNA4 polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EFL4, EPLG4, LERK4, MGC125826 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanPig |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EFNA4 |
|---|---|
| Gene Synonyms | EFL4 EPLG4 LERK4 MGC125826 |
| Gene Full Name | Ephrin-A4 |
| Gene Summary | This gene encodes a member of the ephrin (EPH) family. The ephrins and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. This gene encodes an EFNA class ephrin. Three transcript variants that encode distinct proteins have been identified. |
| Alternative Names | EFL4 EPLG4 LERK4 MGC125826 |
| Molecular Weight(MW) | 24kDa |
| Sequence | 214 amino acids |
Immunoblot
Lane 1: 20ug A549 cell lysateLane 2: 20ug H1437 cell lysateLane 3: 20ug PC3 cell lysatePrimary Antibody Dilution: 1:500Secondary Antibody: Anti-rabbit-HRPSecondary Antibody Dilution: 1:1000Gene Name: EFNA4Submitted by: Erika Mathes Lisabeth, Sanford-Burnham Medical Research Institute
Western blot
Titration: 1.0 ug/mlPositive Control: MCF7 Whole Cell| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
