Western blot
Titration: 1.0 ug/mlPositive Control: Jurkat Whole Cell| Product Name | Rabbit anti-EIF5B polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DKFZp434I036, FLJ10524, IF2, KIAA0741 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | DogHorseHumanPig |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EIF5B |
|---|---|
| Gene Synonyms | DKFZp434I036 FLJ10524 IF2 KIAA0741 |
| Gene Full Name | Eukaryotic translation initiation factor 5B |
| Gene Summary | Accurate initiation of translation in eukaryotes is complex and requires many factors, some of which are composed of multiple subunits. The process is simpler in prokaryotes which have only three initiation factors (IF1, IF2, IF3). Two of these factors are conserved in eukaryotes: the homolog of IF1 is eIF1A and the homolog of IF2 is eIF5B. This gene encodes eIF5B. Factors eIF1A and eIF5B interact on the ribosome along with other initiation factors and GTP to position the initiation methionine tRNA on the start codon of the mRNA so that translation initiates accurately. |
| Alternative Names | DKFZp434I036 FLJ10524 IF2 KIAA0741 |
| Molecular Weight(MW) | 139kDa |
| Sequence | 1220 amino acids |
Western blot
Titration: 1.0 ug/mlPositive Control: Jurkat Whole Cell| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
