Western blot
Host: Rabbit Target Name: HLA-DOB Sample Tissue: HepG2 Whole Cell lysates Antibody Dilution: 1.0ug/ml| Product Name | Rabbit anti-HLA-DOB Antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DOB |
| Immunogen | The immunogen for Anti-HLA-DOB antibody is: synthetic peptide directed towards the N-terminal region of Human HLA-DOB |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogHorseHumanPigRabbitSheep |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HLA-DOB |
|---|---|
| Gene Synonyms | DOB |
| Gene Full Name | major histocompatibility complex, class II, DO beta |
| Gene Summary | HLA-DOB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DOA) and a beta chain (DOB), both anchored in the membrane. It is located in intracellular vesicles. DO suppresses peptide loading of MHC class II molecules by inhibiting HLA-DM. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. |
| Alternative Names | DOB |
| Molecular Weight(MW) | 30kDa |
| Sequence | 273 amino acids |
Western blot
Host: Rabbit Target Name: HLA-DOB Sample Tissue: HepG2 Whole Cell lysates Antibody Dilution: 1.0ug/ml| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
