Western blot
Lane 1: 20ug siRUVBL1 transfected human Saos2 cells Lane 2: 20ug untransfected human Saos2 cells Primary Antibody Dilution : 1:1000 Secondary Antibody:Anti-rabbit-HRP Secondary Antibody Dilution: 1:3000 Color/Signal Descriptions:BAG5 Gene Name:Wenwei Hu, Xuetian Yue, Rutgers Cancer Institute of New Jersey. Submitted by:Western blot
Titration: 1.0 ug/mlPositive Control: Fetal Live| Product Name | Rabbit anti-BAG5 polyclonal antibody - C-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BAG-5 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogHorseHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BAG5 |
|---|---|
| Gene Synonyms | BAG-5 |
| Gene Full Name | BCL2-associated athanogene 5 |
| Gene Summary | The protein encoded by this gene is a member of the BAG1-related protein family. BAG1 is an anti-apoptotic protein that functions through interactions with a variety of cell apoptosis and growth related proteins including BCL-2, Raf-protein kinase, steroid hormone receptors, growth factor receptors and members of the heat shock protein 70 kDa family. This protein contains a BAG domain near the C-terminus, which could bind and inhibit the chaperone activity of Hsc70/Hsp70. Three transcript variants encoding two different isoforms have been found for this gene. |
| Alternative Names | BAG-5 |
| Molecular Weight(MW) | 56kDa |
| Sequence | 488 amino acids |
| Entrez Gene | 9529 |
|---|---|
| SwissProt ID | Q9UL15-2 |
| Protein Accession | NP_001015049 |
Western blot
Lane 1: 20ug siRUVBL1 transfected human Saos2 cells Lane 2: 20ug untransfected human Saos2 cells Primary Antibody Dilution : 1:1000 Secondary Antibody:Anti-rabbit-HRP Secondary Antibody Dilution: 1:3000 Color/Signal Descriptions:BAG5 Gene Name:Wenwei Hu, Xuetian Yue, Rutgers Cancer Institute of New Jersey. Submitted by:
Western blot
Titration: 1.0 ug/mlPositive Control: Fetal Live| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
