Western blot
Titration: 1.0 ug/mlPositive Control: OVCAR-3 Whole Cell| Product Name | Rabbit anti-P4HA1 polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | P4HA |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogHorseHumanMousePigRabbitRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | P4HA1 |
|---|---|
| Gene Synonyms | P4HA |
| Gene Full Name | Prolyl 4-hydroxylase, alpha polypeptide I |
| Gene Summary | This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described. |
| Alternative Names | P4HA |
| Molecular Weight(MW) | 56kDa |
| Sequence | 516 amino acids |
| Entrez Gene | 5033 |
|---|---|
| SwissProt ID | C9JL12 |
| Protein Accession | NP_001136068 |
Western blot
Titration: 1.0 ug/mlPositive Control: OVCAR-3 Whole Cell| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
