Application
Western blot analysis in Ramos cell line lysates (35ug/lane).| Product Name | P4HA1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Prolyl 4-hydroxylase subunit alpha-1, 4-PH alpha-1, Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-1, P4HA1, P4HA |
| Product description | This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described.1) Rose, J. Phd, et al. Mol. Med. (2010) In press : |
| Immunogen | This P4HA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 414-442 amino acids from the C-terminal region of human P4HA1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | P4HA1 |
|---|---|
| Alternative Names | Prolyl 4-hydroxylase subunit alpha-1 4-PH alpha-1 Procollagen-proline 2-oxoglutarate-4-dioxygenase subunit alpha-1 P4HA1 P4HA |
| Molecular Weight(MW) | 61 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western blot analysis in Ramos cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
