Immunohistochemical
Human Hep-2 cellsPrimary Antibody Dilution :1:500Secondary Antibody :Goat anti-rabbit-Alexa Fluor 568Secondary Antibody Dilution :1:400Color/Signal Descriptions :Red: CTPS2 Blue: DAPIGene Name :CTPS2Submitted by :S. John Calise, Edward Chan Lab, University of FloridaWestern blot
Titration: 1.0 ug/mlPositive Control: Hela Whole Cell| Product Name | Rabbit anti-CTPS2 polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DKFZp686C17207, FLJ43358, MGC32997 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | DogGuinea PigHorseMousePigRabbitRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CTPS2 |
|---|---|
| Gene Synonyms | DKFZp686C17207 FLJ43358 MGC32997 |
| Gene Full Name | CTP synthase II |
| Gene Summary | The protein encoded by this gene catalyzes the formation of CTP from UTP with the concomitant deamination of glutamine to glutamate. This protein is the rate-limiting enzyme in the synthesis of cytosine nucleotides, which play an important role in various metabolic processes and provide the precursors necessary for the synthesis of RNA and DNA. Cancer cells that exhibit increased cell proliferation also exhibit an increased activity of this encoded protein. Thus, this protein is an attractive target for selective chemotherapy. Three alternatively spliced transcript variants encoding the same protein have been described for this gene. |
| Alternative Names | DKFZp686C17207 FLJ43358 MGC32997 |
| Molecular Weight(MW) | 64kDa |
| Sequence | 586 amino acids |
Immunohistochemical
Human Hep-2 cellsPrimary Antibody Dilution :1:500Secondary Antibody :Goat anti-rabbit-Alexa Fluor 568Secondary Antibody Dilution :1:400Color/Signal Descriptions :Red: CTPS2 Blue: DAPIGene Name :CTPS2Submitted by :S. John Calise, Edward Chan Lab, University of Florida
Western blot
Titration: 1.0 ug/mlPositive Control: Hela Whole Cell| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
