Immunoblot
Lane 1: 30ug human glioma lysateLane 2: 30ug IL-1 treated human glioma lysatePrimary Antibody Dilution: 1:500Secondary Antibody: Anti-rabbit-HRPSecondary Antibody Dilution: 1:500Gene Name: NLRP3Submitted by: AnonymousWestern blot
Titration: 1.0 ug/mlPositive Control: Fetal Kidney| Product Name | Rabbit anti-NLRP3 polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AGTAVPRL, AII, AII/AVP, AVP, C1orf7, CIAS1, CLR1.1, FCAS, FCU, FLJ95925, MWS, NALP3, PYPAF1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | DogHorseHumanPigRabbit |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NLRP3 |
|---|---|
| Gene Synonyms | AGTAVPRL AII AII/AVP AVP C1orf7 CIAS1 CLR1.1 FCAS FCU FLJ95925 MWS NALP3 PYPAF1 |
| Gene Full Name | NLR family, pyrin domain containing 3 |
| Gene Summary | This gene encodes a pyrin-like protein containing a pyrin domain, a nucleotide-binding site (NBS) domain, and a leucine-rich repeat (LRR) motif. This protein interacts with the apoptosis-associated speck-like protein PYCARD/ASC, which contains a caspase recruitment domain, and is a member of the NALP3 inflammasome complex. This complex functions as an upstream activator of NF-kappaB signaling, and it plays a role in the regulation of inflammation, the immune response, and apoptosis. Mutations in this gene are associated with familial cold autoinflammatory syndrome (FCAS), Muckle-Wells syndrome (MWS), chronic infantile neurological cutaneous and articular (CINCA) syndrome, and neonatal-onset multisystem inflammatory disease (NOMID). |
| Alternative Names | AGTAVPRL AII AII/AVP AVP C1orf7 CIAS1 CLR1.1 FCAS FCU FLJ95925 MWS NALP3 PYPAF1 |
| Molecular Weight(MW) | 114kDa |
| Sequence | 1036 amino acids |
Immunoblot
Lane 1: 30ug human glioma lysateLane 2: 30ug IL-1 treated human glioma lysatePrimary Antibody Dilution: 1:500Secondary Antibody: Anti-rabbit-HRPSecondary Antibody Dilution: 1:500Gene Name: NLRP3Submitted by: Anonymous
Western blot
Titration: 1.0 ug/mlPositive Control: Fetal Kidney| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
