Western blot
Lane 1: 2x107 KIR2DL1 transfected NKL cells IP Antibody :KIR2DL1 Amount of IP Antibody : Primary Antibody :KIR2DL1 Primary Antibody Dilution: 1:250 Secondary Antibody :Anti-rabbit-HRP Secondary Antibody Dilution: 1:10,000 Gene Name :KIR2DL1 Submitted by:Kerry S. Campbell, Institute for Cancer Research.Western blot
Lane 1: 2x107 KIR3DL1 transfected NKL cells IP Antibody :KIR2DL1 Amount of IP Antibody : Primary Antibody :KIR2DL1 Primary Antibody Dilution: 1:250 Secondary Antibody :Anti-rabbit-HRP Secondary Antibody Dilution: 1:10,000 Gene Name :KIR2DL1 Submitted by:Kerry S. Campbell, Institute for Cancer Research.Western blot
Titration: 1.0 ug/mlPositive Control: HepG2 Whole Cell| Product Name | Rabbit anti-KIR2DL1 polyclonal antibody - C-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD158A, KIR-K64, KIR221, NKAT, NKAT1, p58.1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | KIR2DL1 |
|---|---|
| Gene Synonyms | CD158A KIR-K64 KIR221 NKAT NKAT1 p58.1 |
| Gene Full Name | Killer cell immunoglobulin-like receptor, two domains, long cytoplasmic tail, 1 |
| Gene Summary | Killer cell immunoglobulin-like receptors (KIRs) are transmembrane glycoproteins expressed by natural killer cells and subsets of T cells. The KIR genes are polymorphic and highly homologous and they are found in a cluster on chromosome 19q13.4 within the 1 Mb leukocyte receptor complex (LRC). The gene content of the KIR gene cluster varies among haplotypes, although several "framework" genes are found in all haplotypes (KIR3DL3, KIR3DP1, KIR3DL4, KIR3DL2). The KIR proteins are classified by the number of extracellular immunoglobulin domains (2D or 3D) and by whether they have a long (L) or short (S) cytoplasmic domain. KIR proteins with the long cytoplasmic domain transduce inhibitory signals upon ligand binding via an immune tyrosine-based inhibitory motif (ITIM), while KIR proteins with the short cytoplasmic domain lack the ITIM motif and instead associate with the TYRO protein tyrosine kinase binding protein to transduce activating signals. The ligands for several KIR proteins are subsets of HLA class I molecules; thus, KIR proteins are thought to play an important role in regulation of the immune response. |
| Alternative Names | CD158A KIR-K64 KIR221 NKAT NKAT1 p58.1 |
| Molecular Weight(MW) | 38kDa |
| Sequence | 348 amino acids |
Western blot
Lane 1: 2x107 KIR2DL1 transfected NKL cells IP Antibody :KIR2DL1 Amount of IP Antibody : Primary Antibody :KIR2DL1 Primary Antibody Dilution: 1:250 Secondary Antibody :Anti-rabbit-HRP Secondary Antibody Dilution: 1:10,000 Gene Name :KIR2DL1 Submitted by:Kerry S. Campbell, Institute for Cancer Research.
Western blot
Lane 1: 2x107 KIR3DL1 transfected NKL cells IP Antibody :KIR2DL1 Amount of IP Antibody : Primary Antibody :KIR2DL1 Primary Antibody Dilution: 1:250 Secondary Antibody :Anti-rabbit-HRP Secondary Antibody Dilution: 1:10,000 Gene Name :KIR2DL1 Submitted by:Kerry S. Campbell, Institute for Cancer Research.
Western blot
Titration: 1.0 ug/mlPositive Control: HepG2 Whole Cell| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
