Western blot
Titration: 1.0 ug/mlPositive Control: Fetal Lung| Product Name | Rabbit anti-JMY polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FLJ37870, MGC163496, WHDC1L3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | JMY |
|---|---|
| Gene Synonyms | FLJ37870 MGC163496 WHDC1L3 |
| Gene Full Name | Junction mediating and regulatory protein, p53 cofactor |
| Gene Summary | JMY acts both as a nuclear p53/TP53-cofactor and a cytoplasmic regulator of actin dynamics depending on conditions. In nucleus, JMY acts as a cofactor that increases p53/TP53 response via its interaction with p300/EP300. JMY increases p53/TP53-dependent transcription and apoptosis, suggesting an important role in p53/TP53 stress response such as DNA damage. In cytoplasm, JMY acts as a nucleation-promoting factor for both branched and unbranched actin filaments. JMY activates the Arp2/3 complex to induce branched actin filament networks. JMY also catalyzes actin polymerization in the absence of Arp2/3, creating unbranched filaments. JMY contributes to cell motility by controlling actin dynamics. JMY may promote the rapid formation of a branched actin network by first nucleating new mother filaments and then activating Arp2/3 to branch off these filaments. The p53/TP53-cofactor and actin activator activities are regulated via its subcellular location. |
| Alternative Names | FLJ37870 MGC163496 WHDC1L3 |
| Molecular Weight(MW) | 82kDa |
| Sequence | 712 amino acids |
Western blot
Titration: 1.0 ug/mlPositive Control: Fetal Lung| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
