Immunohistochemical
Mouse brain stem cellsPrimary Antibody Dilution :1:500Secondary Antibody :Goat anti-rabbit Alexa-Fluor 594Secondary Antibody Dilution :1:1000Color/Signal Descriptions :EIF3I: Red DAPI:BlueGene Name :EIF3ISubmitted by :Dr. Yuzhi Chen, University of Arkansas for Medical ScienceWestern blot
EIF3I antibody - C-terminal region validated by WB using HepG2 cell lysate at 1.0ug/ml.| Product Name | Rabbit anti-EIF3I polyclonal antibody - C-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EIF3S2, PRO2242, TRIP-1, TRIP1, eIF3-beta, eIF3-p36 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIP |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EIF3I |
|---|---|
| Gene Synonyms | EIF3S2 PRO2242 TRIP-1 TRIP1 eIF3-beta eIF3-p36 |
| Gene Full Name | Eukaryotic translation initiation factor 3, subunit I |
| Gene Summary | EIF3I is a component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. |
| Alternative Names | EIF3S2 PRO2242 TRIP-1 TRIP1 eIF3-beta eIF3-p36 |
| Molecular Weight(MW) | 36kDa |
| Sequence | 325 amino acids |
Immunohistochemical
Mouse brain stem cellsPrimary Antibody Dilution :1:500Secondary Antibody :Goat anti-rabbit Alexa-Fluor 594Secondary Antibody Dilution :1:1000Color/Signal Descriptions :EIF3I: Red DAPI:BlueGene Name :EIF3ISubmitted by :Dr. Yuzhi Chen, University of Arkansas for Medical Science
Western blot
EIF3I antibody - C-terminal region validated by WB using HepG2 cell lysate at 1.0ug/ml.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
