Immunofluorescence
Immunofluorescence analysis of HeLa cells treated with TNF-a 20nM 15', using HSP90B (Phospho-Ser254) Antibody. The picture on the right is treated with the synthesized peptide.Western blot
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using HSP90B (Phospho-Ser254) Antibody. The picture on the right is treated with the synthesized peptide.Western blot analysis of extracts from HeLa cells treated with TNF-a 10ng/ml 30', using HSP90B (Phospho-Ser254) Antibody. The lane on the right is treated with the synthesized peptide| Product Name | Rabbit anti-HSP90B (Phospho-Ser254) polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The antiserum was produced against synthesized peptide derived from human HSP90B around the phosphorylation site of Ser254. |
| Modification | p-Ser254 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WBIHCIFELISA |
| WB:1:500~1:1000 IHC:1:50~1:100 IF:1:100~1:500 ELISA:1:40000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HSP90AB1 |
|---|---|
| Gene Synonyms | HS90B HSP 84 HSP90-beta HSP90AB1 HSPC2 HSPCB Heat shock protein HSP 90-beta |
Immunofluorescence
Immunofluorescence analysis of HeLa cells treated with TNF-a 20nM 15', using HSP90B (Phospho-Ser254) Antibody. The picture on the right is treated with the synthesized peptide.
Western blot
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using HSP90B (Phospho-Ser254) Antibody. The picture on the right is treated with the synthesized peptide.Western blot analysis of extracts from HeLa cells treated with TNF-a 10ng/ml 30', using HSP90B (Phospho-Ser254) Antibody. The lane on the right is treated with the synthesized peptide| Application Notes | WB:1:500~1:1000 IHC:1:50~1:100 IF:1:100~1:500 ELISA:1:40000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
