Immunofluorescence
Immunofluorescence analysis of NIH-3T3 cells, using p38 MAPK (Ab-322) Antibody. The picture on the right is treated with the synthesized peptide.Western blot
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using p38 MAPK (Ab-322) Antibody. The picture on the right is treated with the synthesized peptide.Western blot analysis of extracts from 293 cells, using p38 MAPK (Ab-322) Antibody. The lane on the right is treated with the synthesized peptideWB
(A) Expression of MAPKs and NF-κB in the lung tissues.WB
Effects of total saponins from Dioscorea nipponica Makino and indomethacin on the protein levels of c-Jun, p-c-Jun, MAPK phosphatase, p-MKP, vascular cell adhesion molecule 1, intercellular adhesion molecule 1, C-X-C motif chemokine ligand 1, peroxisome proliferator-activated receptor γ, and Adipor2. All the groups were induced with IL-1β (10 µg/L), while both the total saponins from Dioscorea nipponica Makino (100 µg/L) and indomethacin (100 µg/L) groups were incubated for 72 hours.| Product Name | Rabbit anti-p38 MAPK polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The antiserum was produced against synthesized peptide derived from human p38 MAPK. |
| Host Species | Rabbit |
|---|---|
| Tested Applications | ELISAIFIHCWB |
| WB:1:500~1:1000 IHC:1:50~1:100 IF:1:100~1:500 ELISA:1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MAPK14 |
|---|---|
| Gene Synonyms | protein kinase p38-alpha MAP kinase MXI2 MAP kinase p38 MAPK14 MAX-interacting protein 2 Mitogen-activated protein kinase 14 Mitogen-activated protein kinase p38 MK14 MXI2 |
Immunofluorescence
Immunofluorescence analysis of NIH-3T3 cells, using p38 MAPK (Ab-322) Antibody. The picture on the right is treated with the synthesized peptide.
Western blot
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using p38 MAPK (Ab-322) Antibody. The picture on the right is treated with the synthesized peptide.Western blot analysis of extracts from 293 cells, using p38 MAPK (Ab-322) Antibody. The lane on the right is treated with the synthesized peptide
WB
(A) Expression of MAPKs and NF-κB in the lung tissues.
WB
Effects of total saponins from Dioscorea nipponica Makino and indomethacin on the protein levels of c-Jun, p-c-Jun, MAPK phosphatase, p-MKP, vascular cell adhesion molecule 1, intercellular adhesion molecule 1, C-X-C motif chemokine ligand 1, peroxisome proliferator-activated receptor γ, and Adipor2. All the groups were induced with IL-1β (10 µg/L), while both the total saponins from Dioscorea nipponica Makino (100 µg/L) and indomethacin (100 µg/L) groups were incubated for 72 hours.| Application Notes | WB:1:500~1:1000 IHC:1:50~1:100 IF:1:100~1:500 ELISA:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
