Immunofluorescence
Immunofluorescence analysis of HepG2 cells, using TIE2 (Phospho-Tyr1108) Antibody. The picture on the right is treated with the synthesized peptide.Western blot
Western blot analysis of extracts from NIH-3T3 cells, using TIE2 (Phospho-Tyr1108) Antibody. The lane on the right is treated with the synthesized peptideIF
(A–J) Representative images of staining of the tight junction (green arrow in A) and Tie-2 expression in vascular endothelial cells (green arrow in H) by immunofluorescence staining in different groups.WB
Effects of C16, C16+Tie-2 inhibitor and C16+PI3K inhibitor on the expression of (A) MBP, (B) NF-H, (C) caspase-3, (D) ZO-1, (E) RHOA, (F) Tie-2, (G) AKT, (H) cleaved caspase-3, (I) p-AKT, and (J) pTie-2 were assessed by Western blotting.| Product Name | Rabbit anti-TIE2 (Phospho-Tyr1108) polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The antiserum was produced against synthesized peptide derived from human TIE2 around the phosphorylation site of Tyr1108. |
| Modification | p-Tyr1108 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| WB:1:500~1:1000 IF:1:100~1:500 ELISA:1:10000 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TEK |
|---|---|
| Gene Synonyms | Tyrosine-protein kinase receptor TIE-2 hTIE2 Tyrosine-protein kinase receptor TEK p140 TEK Tunica interna endothelial cell kinase CD202b antigen |
Immunofluorescence
Immunofluorescence analysis of HepG2 cells, using TIE2 (Phospho-Tyr1108) Antibody. The picture on the right is treated with the synthesized peptide.
Western blot
Western blot analysis of extracts from NIH-3T3 cells, using TIE2 (Phospho-Tyr1108) Antibody. The lane on the right is treated with the synthesized peptide
IF
(A–J) Representative images of staining of the tight junction (green arrow in A) and Tie-2 expression in vascular endothelial cells (green arrow in H) by immunofluorescence staining in different groups.
WB
Effects of C16, C16+Tie-2 inhibitor and C16+PI3K inhibitor on the expression of (A) MBP, (B) NF-H, (C) caspase-3, (D) ZO-1, (E) RHOA, (F) Tie-2, (G) AKT, (H) cleaved caspase-3, (I) p-AKT, and (J) pTie-2 were assessed by Western blotting.| Application Notes | WB:1:500~1:1000 IF:1:100~1:500 ELISA:1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
