Application
Western blot analysis of extracts from HepG2 using TIE2 (Phospho-Tyr992) Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | TIE2 (phospho Tyr992) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Angiopoietin-1 receptor,EC=2.7.10.1 Tunica interna endothelial cell kinase Tyrosine-protein kinase receptor TEK Tyrosine-protein kinase receptor TIE-2 hTIE2 p140 TEK CD202b TEK TIE2 |
| Product description | The TEK receptor tyrosine kinase is expressed almost exclusively in endothelial cells in mice, rats, and humans. This receptor possesses a unique extracellular domain containing 2 immunoglobulin-like loops separated by 3 epidermal growth factor-like repeats that are connected to 3 fibronectin type III-like repeats. The ligand for the receptor is angiopoietin-1. Defects in TEK are associated with inherited venous malformations; the TEK signaling pathway appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis. TEK is closely related to the TIE receptor tyrosine kinase.1) Schooley K.A., Baum P.R. Oncogene 8:663-670(1993) |
| Immunogen | TIE2 (phospho Tyr992) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 992 (E-V-Y (p)-V-K) derived from Human TIE2. |
| Modification | p-Tyr992 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TEK |
|---|---|
| Alternative Names | Angiopoietin-1 receptor EC=2.7.10.1 Tunica interna endothelial cell kinase Tyrosine-protein kinase receptor TEK Tyrosine-protein kinase receptor TIE-2 hTIE2 p140 TEK CD202b TEK TIE2 |
| Molecular Weight(MW) | 126 kDa |
| Tissue Specificity | The Antibody detects endogenous levels of TIE2 only when phosphorylated at Tyr992. |
| Entrez Gene | 7010 |
|---|---|
| Protein Accession | NP_000450.2 |
Application
Western blot analysis of extracts from HepG2 using TIE2 (Phospho-Tyr992) Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
