Dot blot
Dot blot analysis of Rabbit anti-Phospho-MeCP2(S421) polyclonal antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.| Product Name | Rabbit anti-Phospho-MeCP2(S421) polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MeCp-2 protein |
| Product description | This MeCP2 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S421 of human MeCP2. |
| Immunogen | synthetic phosphopeptide corresponding to amino acid residues surrounding S421 of human MeCP2 |
| Modification | p-S421 |
| Modification Notes | synthetic phosphopeptide corresponding to amino acid residues surrounding S421 of human MeCP2 |
| Modificaiton Site | S421 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Clone Number | E21 |
| Host Species | Rabbit |
| Tested Applications | DB |
| DB:1:500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MECP2 |
|---|---|
| Gene Synonyms | MECP2 Methyl-CpG-binding protein 2 |
| Gene Full Name | MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of most cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females. [provided by RefSeq, Jul 2009] |
| Target | Methyl-CpG-binding protein 2 |
| Alternative Names | MeCp-2 protein |
| Molecular Weight(MW) | 52441Da |
| Function | Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A |
| Sequence Similarities | Contains 2 A.T hook DNA-binding domains. Contains 1 MBD (methyl-CpG-binding) domain. |
| Tissue Specificity | Present in all adult somatic tissues tested. |
| Cellular Localization | Nucleus. Note=Colocalized with methyl-CpG in the genome |
Dot blot
Dot blot analysis of Rabbit anti-Phospho-MeCP2(S421) polyclonal antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.| Application Notes | DB:1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification. |
