Application
Immunohistochemistry staining of MECP2 in testis tissue using MECP2 Antibody.| Product Name | MECP2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA. MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases. In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract. |
| Immunogen | MECP2 antibody was raised against amino acids 11 - 25 of MECP2 (Human). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| MECP2 antibody can be used in ELISA starting at 1:10000, Western Blot starting at 1:500 - 1:2000, immunohistochemistry starting at 1:50, immunofluorescence starting at 1:200 - 1:1000, and flow cytometry starting at 1:200 - 1:400. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | MECP2 |
|---|---|
| Alternative Names | MECP2 AUTSX3 Methyl-CpG-binding protein 2 MRX16 MRX79 MRXSL RTT RTS MeCp-2 protein RS MRXS13 PPMX |
Application
Immunohistochemistry staining of MECP2 in testis tissue using MECP2 Antibody.| Application Notes | MECP2 antibody can be used in ELISA starting at 1:10000, Western Blot starting at 1:500 - 1:2000, immunohistochemistry starting at 1:50, immunofluorescence starting at 1:200 - 1:1000, and flow cytometry starting at 1:200 - 1:400. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | MECP2 antibody can be stored short term 4 ˚C. For long term storage aliquot and store at -20 ˚C. As with all antibodies avoid freeze/thaw cycles. |
| Storage Buffer | PBS, 0.2% gelatin, 0.05% sodium azide. |
