WB
connexin 40: Western blot analysis of connexin 40 expression in CCD-1064Sk (A) and Hs68 (B) whole cell lysates| Product Name | Rabbit anti-connexin 40 polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | connexin 40 is a rabbit polyclonal antibodycorresponding to amino acids mappingwithin a C-terminalcytoplasmic domain ofconnexin 40 of human origin. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIPWB |
| WB:1:200-1:1000 IP:1-2 µg per 100-500 µg of total protein (1ml ofcell lysate) IF:1:50-1:500 ELISA:1:30-1:3000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | connexin 40 |
|---|---|
| Gene Full Name | connexons |
| Gene Summary | The connexin family of proteins form hexameric complexes called “connexons” that facilitate movement of low molecular weight proteins between cells via gap junctions. Connexin proteins share a common topology of four transmembrane α-helical domains, two extracellular loops, a cytoplasmic loop and cytoplasmic N- and C-termini. Many of the key functional differences arise from specific amino-acid substitutions in the most highly conserved domains, the transmembrane and extracellular regions. Each of the approximately 20 connexin isoforms produces channels with distinct permeabilities and electrical and chemical sensitivities; therefore, one connexin usually cannot fully substitute for another. Consequently, a wide variety of malignant phenotypes associate with decreased connexin expression and gap junction communication, dependent on the particular connexin that is affected. For example, upregulation of connexin 40 following cardiac surgery can mark a susceptibility to post-operative atrial fibrillation. |
| Molecular Weight(MW) | 40 kDa |
| Cellular Localization | Geneticlocus: GJA5 (human) mapping to 1q21.2; Gja5 (mouse) mapping to 3 F2.1. |
WB
connexin 40: Western blot analysis of connexin 40 expression in CCD-1064Sk (A) and Hs68 (B) whole cell lysates| Application Notes | WB:1:200-1:1000 IP:1-2 µg per 100-500 µg of total protein (1ml ofcell lysate) IF:1:50-1:500 ELISA:1:30-1:3000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4° C |
| Storage Buffer | PBSwith < 0.1% sodiumazide and 0.1% gelatin. |
