Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing cytoplasmic staining of cells in seminiferous ducts and Leydig cells.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Western blot analysis of GPx-4 expression in mouse testis tissue extract.Application
Western blot analysis of GPx-4 expression in 293T whole cell lysate.Application
Western blot analysis of GPx-4 expression in non-transfected: (A) and human GPx-4 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of GPx-4 expression in mouse testis (A) and rat testis (B) tissue extracts.| Product Name | GPx-4 Antibody (H-90) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 108-197 mapping at the C-terminus of GPx-4 of human origin |
| Modification Notes | GPx-4, also known as phospholipid hydroperoxide glutathione peroxidase (PHGPx), is the only known antioxidant enzyme that reduces phospholipid hydroperoxides within membranes and lipoproteins, thus inhibiting lipid peroxidation. A number of pathophysiological states rely on peroxidation of lipids, suggesting that GPx-4 plays a crucial role in antioxidative defense. GPx-4 is expressed at low levels in a wide variety of organs with two distinct forms: L-GPx-4, which localizes in the mitochondria, and S-GPx-4, the cytosolic form. In some tissues, GPx-4 is more highly expressed, suggesting that GPx-4 is involved in more specific functions. For example, regulation of the enzyme in testicular tissue implies a necessary role for GPx-4 in sperm maturation. The gene encoding GPx-4 presents a number of different protein-binding domains, allowing regulation of expression to be influenced by Sp1, NF-Y and ApoER2, as well as other proteins. Therefore, complex interactions between a variety of proteins and the GPx-4 gene, in addition to interplay with fatty acids, cytokines and antioxidants, ultimately dictate the functional significance of GPx-4 . |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of GPx-4 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 108-197 mapping at the C-terminus of GPx-4 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 108-197 mapping at the C-terminus of GPx-4 of human origin |
| Entrez Gene | 2879 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing cytoplasmic staining of cells in seminiferous ducts and Leydig cells.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Western blot analysis of GPx-4 expression in mouse testis tissue extract.
Application
Western blot analysis of GPx-4 expression in 293T whole cell lysate.
Application
Western blot analysis of GPx-4 expression in non-transfected: (A) and human GPx-4 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of GPx-4 expression in mouse testis (A) and rat testis (B) tissue extracts.| Application Notes | recommended for detection of GPx-4 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
