Application
Western blot analysis of PNK expression in non-transfected 293T: (A), human PNK transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of PNK expression in non-transfected: (A) and human PNK transfected: (B) 293T whole cell lysates and HeLa nuclear extract (C).Application
Western blot analysis of PNK expression in non-transfected: (A) and human PNK transfected: (B) 293T whole cell lysates.| Product Name | PNK Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 222-521 mapping at the C-terminus of PNK of human origin |
| Modification Notes | Bifunctional polynucleotide phosphatase/kinase (PNK), also referred to as polynucleotide kinase-3'-phosphatase, is a member of the DNA 3’ phosphatase family. PNK is the protein expressed by the gene PNKP and contains a 3'-phosphatase domain with similarity to L-2-haloacid dehalogenases and a reported ATP binding site. PNK is a nuclear protein that is involved in DNA repair following damage caused by radiation or oxidation. The protein catalyzes the phosphorylation of DNA at the hydroxy termini but can also dephosphorylate its 3’-phosphate termini. The highest levels of expression of PNK occur in testis, pancreas, spleen, kidney and heart. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of PNK of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 222-521 mapping at the C-terminus of PNK of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 222-521 mapping at the C-terminus of PNK of human origin |
| Entrez Gene | 11284 |
|---|
Application
Western blot analysis of PNK expression in non-transfected 293T: (A), human PNK transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of PNK expression in non-transfected: (A) and human PNK transfected: (B) 293T whole cell lysates and HeLa nuclear extract (C).
Application
Western blot analysis of PNK expression in non-transfected: (A) and human PNK transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of PNK of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
