Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human liver tissue showing cytoplasmic staining of hepatocytes.Application
Western blot analysis of Cyclophilin D expression in non-transfected: (A) and mouse Cyclophilin D transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Cyclophilin D expression in JAR (A) and NIH/3T3 (B) whole cell lysates.| Product Name | Cyclophilin D Antibody (H-185) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 186-370 mapping at the C-terminus of Cyclophilin D of human origin |
| Modification Notes | Cyclophilins are conserved, ubiquitous and abundant cytosolic peptidyl-prolyl cis-trans isomerases that accelerate the isomerization of XaaPro peptide bonds and the refolding of proteins. Human cyclophilin A (CyPA), an intracellular protein of 165 amino acids, is the target of cyclosporin A (CsA) and is encoded by a single unique gene conserved from yeast to humans. Cyclophilin B (CyPB) is secreted in biological fluids such as blood or milk and binds to a specific receptor present on the human lymphoblastic cell line Jurkat and on human peripheral blood lymphocytes. Cyclophilin D (CyP40) is a widely expressed cytoplasmic protein that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. It is a widely expressed cytoplasmic protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Cyclophilin D of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 186-370 mapping at the C-terminus of Cyclophilin D of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 186-370 mapping at the C-terminus of Cyclophilin D of human origin |
| Entrez Gene | 5481 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human liver tissue showing cytoplasmic staining of hepatocytes.
Application
Western blot analysis of Cyclophilin D expression in non-transfected: (A) and mouse Cyclophilin D transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Cyclophilin D expression in JAR (A) and NIH/3T3 (B) whole cell lysates.| Application Notes | recommended for detection of Cyclophilin D of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
