Application
Western blot analysis of UGT1A7 expression in non-transfected: (A) and human UGT1A7 transfected: (B) 293T whole cell lysates and human liver tissue extract (C).Application
Western blot analysis of UGT1A expression in rat pituitary (A), mouse small intestine (B) and rat liver (C) tissue extracts.Application
Western blot analysis of UGT1A expression in rat pituitary tissue extract.Application
Western blot analysis of UGT1A expression in non-transfected 293T: (A) and human UGT1A transfected 293T: (B) whole cell lysates and rat liver tissue extract (C).Application
Western blot analysis of UGT1A expression in non-transfected: (A) and human UGT1A transfected: (B) 293 whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human liver tissue showing cytoplasmic staining of hepatocytes at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic staining.Application
Western blot analysis of UGT1A expression in rat liver (A) and mouse liver (B) tissue extracts.| Product Name | UGT1A Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 234-533 mapping at the C-terminus of UGT1A1 of human origin |
| Modification Notes | Glucuronidation, an important bile acid detoxification pathway, is catalyzed by enzymes belonging to the UDP-glucuronosyltransferase (UGT) superfamily. UGT genes are classified into the UGT1A and UGT2B subfamilies. Although each subfamily and each isoform shows tissue-specific patterns of distribution, the underlying mechanisms for this tissue specificity have not been fully elucidated. The human UDP-glucuronosyltransferase 1 (UGT1) locus encodes at least ten UGT1A proteins (UGT1A1-UGT1A10) that play a prominent role in drug and xenobiotic metabolism. Research indicates that nuclear receptors such as pregnane X receptor (PXR), constitutive androstane receptor (CAR) and peroxisome proliferator-activated receptor (PPAR) can regulate UGTs, which may contribute to the tissue-specific expression pattern of UGTs. Deficiency in the expression and/or activity of UGTs may lead to genetic and acquired diseases such as Crigler-Najjar syndrome and Gilbert syndrome. Based on their ability to catalyze the glucuronidation of xenobiotics and endobiotics, UGTs play a critical role in hormonal homeostasis, energy metabolism, bilirubin clearance and xenobiotic detoxification. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of UGT1A family members of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 234-533 mapping at the C-terminus of UGT1A1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 234-533 mapping at the C-terminus of UGT1A1 of human origin |
| Entrez Gene | 54658 |
|---|
Application
Western blot analysis of UGT1A7 expression in non-transfected: (A) and human UGT1A7 transfected: (B) 293T whole cell lysates and human liver tissue extract (C).
Application
Western blot analysis of UGT1A expression in rat pituitary (A), mouse small intestine (B) and rat liver (C) tissue extracts.
Application
Western blot analysis of UGT1A expression in rat pituitary tissue extract.
Application
Western blot analysis of UGT1A expression in non-transfected 293T: (A) and human UGT1A transfected 293T: (B) whole cell lysates and rat liver tissue extract (C).
Application
Western blot analysis of UGT1A expression in non-transfected: (A) and human UGT1A transfected: (B) 293 whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human liver tissue showing cytoplasmic staining of hepatocytes at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic staining.
Application
Western blot analysis of UGT1A expression in rat liver (A) and mouse liver (B) tissue extracts.| Application Notes | recommended for detection of UGT1A family members of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
