Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human colon tissue showing cytoplasmic staining of glandular cells at high magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of TGFβ RI expression in T84 whole cell lysate.Application
Western blot analysis of TGFβ RI expression in IMR-32 whole cell lysate.Application
Western blot analysis of ALK-1 expression in non-transfected: (A), human ALK-1 transfected: (B) and human platelet (C) 293T whole cell lysates.Application
Western blot analysis of TGFβ RI expression in SK-N-SH whole cell lysate.| Product Name | TGFβ RI Antibody (T-19) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | raised against a peptide mapping within a C-terminal cytoplasmic domain of TGFβ RI of human origin |
| Modification Notes | A total of three members of the TGF∫ family, namely TGF∫1, TGF∫2 and TGF∫3, have been identified in mammals. Each is synthesized as a latent precursor that is subsequently cleaved forming the 112 amino acid growth factor which becomes active upon dimerization. TGF∫s mediate their activity by high affinity binding to the TGF∫ receptor type-II (TGF∫ RII) with a cytoplasmic serine-threonine kinase domain. For signaling growth inhibition and early gene responses, TGF∫ RII requires both its kinase activity and its association with a TGF∫-binding protein, designated TGF∫ receptor type-1 (TGF∫ RI). TGF∫ RI is a 503 amino acid single-pass type I membrane protein that is expressed ubiquitously and, with TGF∫ RII, functions as a receptor for TGF∫. Defects in the gene encoding TGF∫ RI are the cause of aortic aneurysm familial thoracic type 5 (AAT5), Loeys-Dietz syndrome type 2A (LDS2A) and Loeys-Dietz syndrome type 1A (LDS1A). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for the detection of the TGFβ superfamily type I receptors ALK-1, ACTR-I, TGFβ RI and, to a lesser extent ACTR-IB of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | raised against a peptide mapping within a C-terminal cytoplasmic domain of TGF&beta RI of human origin |
|---|---|
| Tissue Specificity | raised against a peptide mapping within a C-terminal cytoplasmic domain of TGFβ RI of human origin |
| Entrez Gene | 90 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human colon tissue showing cytoplasmic staining of glandular cells at high magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of TGFβ RI expression in T84 whole cell lysate.
Application
Western blot analysis of TGFβ RI expression in IMR-32 whole cell lysate.
Application
Western blot analysis of ALK-1 expression in non-transfected: (A), human ALK-1 transfected: (B) and human platelet (C) 293T whole cell lysates.
Application
Western blot analysis of TGFβ RI expression in SK-N-SH whole cell lysate.| Application Notes | recommended for the detection of the TGFβ superfamily type I receptors ALK-1, ACTR-I, TGFβ RI and, to a lesser extent ACTR-IB of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
