Application
Western blot analysis of human recombinant Twinfilin-1 fusion protein.| Product Name | Twinfilin-1 Antibody (H-62) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 175-236 mapping near the C-terminus of Twinfilin-1 of human origin |
| Modification Notes | Twinfilin is a highly conserved Actin monomer-binding protein that regulates cytoskeletal dynamics in organisms from yeast to mammals. Twinfilin is composed of two ADF-homology domains; it coordinates filament severing and monomer sequestering at sites of rapid Actin turnover, thus preventing assembly of the monomer into filaments. Twinfilin-1 is the mammalian homolog and is expressed in embryos and in most adult non-muscle cell types. Twinfilin-1 binds ADP-G-Actin and efficiently halts Actin filament assembly by inhibiting the nucleotide exchange on Actin monomers and directly interacting with the capping protein. Phosphatidylinositol (4,5)-bisphosphate inhibits the activity of Twinfilin-1, while two small GTPases, namely Rac1 and Cdc42, induce the redistribution of Twinfilin-1 to membrane ruffles and cell-cell contacts, respectively. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Twinfilin-1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 175-236 mapping near the C-terminus of Twinfilin-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 175-236 mapping near the C-terminus of Twinfilin-1 of human origin |
| Entrez Gene | 5756 |
|---|
Application
Western blot analysis of human recombinant Twinfilin-1 fusion protein.| Application Notes | recommended for detection of Twinfilin-1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
