Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human spleen tissue showing membrane staining of cells in white pulp.Application
Western blot analysis of MHC class I expression in human PBL whole cell lysate.Application
Western blot analysis of MHC class I expression in CCRF-CEM whole cell lysate.Application
Western blot analysis of MHC class I expression in HL-60 whole cell lysate.Application
Western blot analysis of HLA-B expression in non-transfected: (A) and human HLA-B transfected: (B) 293T whole cell lysates.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane localization.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human lymphoma tissue showing membrane localization.Application
Western blot analysis of MHC class I expression in Raji whole cell lysate.| Product Name | MHC class I Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 63-362 of MHC class I of human origin |
| Modification Notes | Major histocompatibility complex (MHC) molecules, also designated human leukocyte antigen (HLA) molecules, are cell-surface receptors that bind foreign peptides and present them to T lymphocytes. MHC class I molecules consist of two polypeptide chains, an a or heavy chain, and ∫-2-Microglobulin, a non-covalently associated protein. Cytotoxic T lymphocytes bind antigenic peptides presented by MHC class I molecules. Antigens that bind to MHC class I molecules are typically 8-10 residues in length and are stabilized in a peptide binding groove. MHC class II molecules are encoded by polymorphic MHC genes and consist of a non-covalent complex of an a and b chain. Helper T lymphocytes bind antigenic peptides presented by MHC class II molecules. MHC class II molecules bind 13-18 amino acid antigenic peptides. Accumulating in endosomal/lysosomal compartments and on the surface of B cells, HLA-DM and -DO molecules regulate binding of exogenous peptides to class II molecules (HLA-DR) by sustaining a conformation that favors peptide exchange. The differential structural properties of MHC class I and class II molecules account for their respective roles in activating different populations of T lymphocytes. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of MHC class I of human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 63-362 of MHC class I of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 63-362 of MHC class I of human origin |
| Entrez Gene | 3106 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human spleen tissue showing membrane staining of cells in white pulp.
Application
Western blot analysis of MHC class I expression in human PBL whole cell lysate.
Application
Western blot analysis of MHC class I expression in CCRF-CEM whole cell lysate.
Application
Western blot analysis of MHC class I expression in HL-60 whole cell lysate.
Application
Western blot analysis of HLA-B expression in non-transfected: (A) and human HLA-B transfected: (B) 293T whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane localization.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human lymphoma tissue showing membrane localization.
Application
Western blot analysis of MHC class I expression in Raji whole cell lysate.| Application Notes | recommended for detection of MHC class I of human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
