Application
Immunofluorescence staining of normal mouse intestine frozen section showing membrane staining.Application
Western blot analysis of MYH expression in rat skeletal muscle tissue extract.| Product Name | MYH Antibody (H-300) AC |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1641-1940 mapping at the C-terminus of Myosin heavy chain of human origin |
| Modification Notes | Myosin is a highly conserved, ubiquitously expressed protein that interacts with Actin to generate the force for cellular movements. Conventional Myosins are hexameric proteins consisting of two heavy chain subunits, a pair of non-phosphorylatable light chain subunits and a pair of phosphorylatable light chain subunits. Three general classes of Myosin have been cloned: smooth muscle Myosins, striated muscle Myosins and non-muscle Myosins. Contractile activity in smooth muscle is regulated by the calcium/calmodulin-dependent phosphorylation of Myosin light chain (MLC) by Myosin light chain kinase. Myosin heavy chains, which are encoded by the MYH gene family, contain Actin-activated ATPase activity which generates the motor function of Myosin. Myosin heavy chains were initially isolated from a human fetal skeletal muscle and are the major determinant in the speed of contraction of skeletal muscle. Various isoforms of myosin heavy chains are differentially expressed depending on the functional activity of the muscle. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of skeletal and cardiac Myosin heavy chain of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1641-1940 mapping at the C-terminus of Myosin heavy chain of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1641-1940 mapping at the C-terminus of Myosin heavy chain of human origin |
| Entrez Gene | 4619 |
|---|
Application
Immunofluorescence staining of normal mouse intestine frozen section showing membrane staining.
Application
Western blot analysis of MYH expression in rat skeletal muscle tissue extract.| Application Notes | recommended for detection of skeletal and cardiac Myosin heavy chain of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
