Application
Western blot analysis of STAM expression in non-transfected 293T: (A), mouse STAM transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of STAM expression in HeLa (A), MCF7 (B) and Jurkat (C) whole cell lysates.| Product Name | STAM Antibody (H-175) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 366-540 mapping at the C-terminus of STAM of human origin |
| Modification Notes | Cytokine stimulation of the IL-2 receptor leads to the tyrosine phosphorylation of a number of cellular proteins and to the induction of various transcription factors including c-Fos and c-Myc (1-5). The signal transducing adapter molecule, STAM, is speculated to play a role in c-Myc induction by various cytokines (6). STAM contains an SH3 (Src homology 3) motif as well as an immunoreceptor tyrosine-based activation (ITAM) motif, both of which appear to be required for c-Myc induction in response to IL-2 and GM-CSF (6,7). STAM associates with JAK3 and JAK2 via its ITAM region, and it is tyrosine phosphorylated by JAK3 and JAK2 after stimulation with IL-2 and GM-CSF, respectively (7). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of STAM of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 366-540 mapping at the C-terminus of STAM of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 366-540 mapping at the C-terminus of STAM of human origin |
| Entrez Gene | 8027 |
|---|
Application
Western blot analysis of STAM expression in non-transfected 293T: (A), mouse STAM transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of STAM expression in HeLa (A), MCF7 (B) and Jurkat (C) whole cell lysates.| Application Notes | recommended for detection of STAM of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
