Application
Western blot analysis of IL-11 expression in non-transfected 293T: (A), human IL-11 transfected 293T: (B), An3 CA (C) and NIH/3T3 (D) whole cell lysates.Application
Western blot analysis of IL-11 expression in An3 CA whole cell lysate.Application
Western blot analysis of IL-11 expression in non-transfected: (A) and human IL-11 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of human recombinant IL-11.| Product Name | IL-11 Antibody (H-169) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 31-199 of IL-11 of human origin |
| Modification Notes | Interleukin-11, or IL-11, is a pleiotropic cytokine that is biologically related to IL-6, leukemia inhibitory factor (LIF), oncostatin M (OSM) and ciliary neurotrophic factor (CNTF). IL-11 is a stromal cell-derived cytokine which stimulates the proliferation of primitive hematopoietic progenitor cells and, together with Flt 3-L, stimulates the expansion of CD34+ cell populations. Human IL-11 cDNA encodes a 199 amino acid precursor with a 21 amino acid hydrophobic signal peptide which is cleaved to generate a glycosylated biologically active form. IL-11 exerts its biological effects through the interleukin-11 receptor, IL-11R, which is composed of an IL-11 receptor-specific chain designated IL-11Rå, and gp130, the signal transducing component common to the IL-6, LIF, OSM and CNTF receptors. Stimulation of the IL-11R results in the activation of the Janus tyrosine kinase family members JAK1 and JAK2 which, once activated, induce the nuclear translocation of the transcription factors Stat1 and Stat3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of IL-11 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 31-199 of IL-11 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 31-199 of IL-11 of human origin |
| Entrez Gene | 3589 |
|---|
Application
Western blot analysis of IL-11 expression in non-transfected 293T: (A), human IL-11 transfected 293T: (B), An3 CA (C) and NIH/3T3 (D) whole cell lysates.
Application
Western blot analysis of IL-11 expression in An3 CA whole cell lysate.
Application
Western blot analysis of IL-11 expression in non-transfected: (A) and human IL-11 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of human recombinant IL-11.| Application Notes | recommended for detection of IL-11 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
