Application
Western blot analysis of β3-AR expression in C6 (A) and EOC 20 (B) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing membrane localization.| Product Name | β3-AR Antibody (M-50) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 351-400 mapping within a C-terminal cytoplasmic domain of β3-AR of mouse origin |
| Modification Notes | Beta 3-adrenergic receptors (b3-ARs) bind cathecholamines (epinephrine, norepinephrine), and primarily regulate lipolysis and thermogenesis in adipose. b3-ARs are present in adipose tissues, heart, and in smooth muscle of bladder, colon, small intestine, and stomach. The human corpus cavernosum exhibits basal b3-AR-mediated vasorelaxant tone and activity is linked to inhibition of the RhoA/Rho-kinase pathway. b3-AR interacts directly with the SH3 domain of Src through proline-rich motifs (PXXP) in the third intracellular loop and the carboxy-terminus. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of β3-AR of mouse and rat origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 351-400 mapping within a C-terminal cytoplasmic domain of &beta 3-AR of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 351-400 mapping within a C-terminal cytoplasmic domain of β3-AR of mouse origin |
| Entrez Gene | 11556 |
|---|
Application
Western blot analysis of β3-AR expression in C6 (A) and EOC 20 (B) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing membrane localization.| Application Notes | recommended for detection of β3-AR of mouse and rat origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
