Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing cytoskeletal localization.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and cytoskeletal localization.Application
Western blot analysis of Tropomyosin α expression in non-transfected 293T: (A), human Tropomyosin α transfected 293T: (B) and CCD-1064Sk (C) whole cell lysates.Application
Western blot analysis of Tropomyosin expression in non-transfected 293T: (A), mouse Tropomyosin transfected 293T: (B), CCD-1064Sh (C) and Sol8 (D) whole cell lysates.Application
Western blot analysis of Tropomyosin expression in non-transfected 293T: (A), mouse Tropomyosin transfected 293T: (B), CCD-1064Sh (C) and Sol8 (D) whole cell lysates.Application
Western blot analysis of Tropomyosin expression in Sol8 whole cell lysate.Application
Western blot analysis of Tropomyosin expression in CCD-1064Sk whole cell lysate.Application
Western blot analysis of Tropomyosin expression in rat skeletal muscle tissue extract.Application
Western blot analysis of Tropomyosin expression in HeLa (A) and MIA PaCa-2 (B) whole cell lysates.| Product Name | Tropomyosin Antibody (FL-284) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-284 representing full length Tropomyosin α of human origin |
| Modification Notes | Tropomyosins are a group of structural proteins. Tropomyosins are present in virtually all eukaryotic cells, both muscle and non-muscle, where they bind Actin filaments and function to modulate Actin-Myosin interaction and stabilize Actin filament structure. Tropomyosin å is encoded by the TPM1 gene, which maps to human chromosome 15q22.1 and undergoes alternative splicing to generate at least four isoforms, including skeletal muscle (isoform 1), smooth muscle (isoform 2), fibroblast/TM3 (isoform 3) and isoform 4. Tropomyosin ∫ is encoded by the TPM2 gene, which maps to human chromosome 9p13.3 and undergoes alternative splicing to generate three isoforms, including skeletal muscle (isoform 1), non-muscle/fibroblast TM36/epithelial TMe1 (isoform 2) and non-muscle (isoform 3). Troponin I binds Tropomyosin at a specific region and the association of Tropomyosin-Troponin with Actin filaments may increase the rigidity of Actin filaments. Tropomyosin also interacts with Caldesmon to regulate smooth muscle contraction. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Tropomyosin α isoforms 1-4, Tropomyosin β isoforms 1-3, Tropomyosin γ and Tropomyosin 4 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-284 representing full length Tropomyosin &alpha of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-284 representing full length Tropomyosin α of human origin |
| Entrez Gene | 7168 |
|---|
Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing cytoskeletal localization.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and cytoskeletal localization.
Application
Western blot analysis of Tropomyosin α expression in non-transfected 293T: (A), human Tropomyosin α transfected 293T: (B) and CCD-1064Sk (C) whole cell lysates.
Application
Western blot analysis of Tropomyosin expression in non-transfected 293T: (A), mouse Tropomyosin transfected 293T: (B), CCD-1064Sh (C) and Sol8 (D) whole cell lysates.
Application
Western blot analysis of Tropomyosin expression in non-transfected 293T: (A), mouse Tropomyosin transfected 293T: (B), CCD-1064Sh (C) and Sol8 (D) whole cell lysates.
Application
Western blot analysis of Tropomyosin expression in Sol8 whole cell lysate.
Application
Western blot analysis of Tropomyosin expression in CCD-1064Sk whole cell lysate.
Application
Western blot analysis of Tropomyosin expression in rat skeletal muscle tissue extract.
Application
Western blot analysis of Tropomyosin expression in HeLa (A) and MIA PaCa-2 (B) whole cell lysates.| Application Notes | recommended for detection of Tropomyosin α isoforms 1-4, Tropomyosin β isoforms 1-3, Tropomyosin γ and Tropomyosin 4 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
