Application
Western blot analysis of AF9 expression in HL-60 whole cell lysate.| Product Name | AF9 Antibody (B-21) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of AF9 of human origin by WB, IP and ELISA |
| Modification Notes | The MLL (ALL-1, HRX) gene influences myelomonocytic differentiation, and different chromosomal translocations can result in a range of MLL fusion proteins that mediate leukemia. Frequent translocation partners of MLL include ELL, ENL, AF4, AF6 and AF9. ELL (elongation factor RNA polymerase II, Men) encodes an RNA polymerase II elongation factor that is implicated in t(11;19)(q23;p13.1) translocation in myeloid leukemias. AF9 (MLLT3, YEATS3) fusion with the MLL gene results in a t[(9;11)(p22;q23)] translocation, which is associated with de novo acute myelogenous leukemia (AML). ENL (MLLT1, LTG19, YEATS1, eleven-nineteen leukemia protein) is capable of activating transcription from synthetic reporter genes in both lymphoid and myeloid cells. The t[(11;19) (q23;p13)] translocation results in the MLL-ENL fusion protein, which is commonly found in infant acute leukemias of both the myeloid and lymphoid lineage. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of AF9 of human origin by WB IP and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of AF9 of human origin by WB, IP and ELISA |
| Entrez Gene | 4300 |
|---|
Application
Western blot analysis of AF9 expression in HL-60 whole cell lysate.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
