Application
Western blot analysis of TFEB expression in non-transfected: (A) and human TFEB transfected: (B) 293T whole cell lysates.IF
(C) Immunostaining of CD68 (red), TFEB (green), and DAPI (blue) in mouse carotid atherosclerotic lesions (n = 3:4:4), scale bar = 100 μm.| Product Name | TFEB Antibody (H-125) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The DNA-binding factor TFE3 contains adjacent helix-loop-helix (HLH) and leucine zipper (LZ) domains flanked by an upstream basic region. These protein motifs are frequently observed in other transcription factors and are particularly common to members of the Myc family. TFE3 is ubiquitously expressed and can directly associate with DNA as either a homodimer or a heterodimer formed with two related proteins, TFEB or TFEC. TFE3 binds to and activates the microE3 motif of the immunoglobulin heavy-chain enhancer to induce B cell-specific gene transcription and DNA recombination. TFEB binds to the major late promoter of adenovirus and specifically associates with DNA as both a homodimer and a heterodimer with TFE3. TFEB is expressed at low levels in the embryo but at high levels in the trophoblast cells of the placenta, where it plays a critical role in regulating normal vascularization of the placenta. TFEC shares a bHLH/LZ structure with TFE3 and a closely related protein microphthalmia-associated transcription factor (MITF), which is critically involved in melanocyte differentiation. Unlike TFE3, the expression of TFEC is largely restricted to fibroblasts, myoblasts, chondrosarcoma cells and myeloma cells. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ChIPELISAGSIFIPWB |
| recommended for detection of TFEB of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 326-450 mapping near the C-terminus of TFEB of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 326-450 mapping near the C-terminus of TFEB of human origin |
| Entrez Gene | 7942 |
|---|
Application
Western blot analysis of TFEB expression in non-transfected: (A) and human TFEB transfected: (B) 293T whole cell lysates.
IF
(C) Immunostaining of CD68 (red), TFEB (green), and DAPI (blue) in mouse carotid atherosclerotic lesions (n = 3:4:4), scale bar = 100 μm.| Application Notes | recommended for detection of TFEB of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
