Application
Western blot analysis of E2ig5 expression in non-transfected: (A) and mouse E2ig5 transfected: (B) 293T whole cell lysates.| Product Name | HGTD-P Antibody (C-15) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping near the C-terminus of HGTD-P of human origin |
| Modification Notes | HGTD-P (human growth and transformation-dependent protein), also known as E2IG5 or FAM162A, is a 154 amino acid single-pass membrane protein belonging to the UPF0389 family. Considered a proapoptotic protein, HGTD-P is an effector of cell death induced by hypoxia-ischemia (HI) and is therefore considered a potential target in treating HI-induced brain damage. HGTD-P localizes to the mitochondria and, when overexpressed, induces the mitochondrial permeability transition by interacting with voltage dependent anion channels. HGTD-P facilitates apoptotic cell death via the mitochondrial apoptotic cascades, including permeability transition, cytochrome c release and caspase 9 activation. HGTD-P is regulated and activated by HIF-1å through a hypoxia-responsive element on the HGTD-P promoter region. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of HGTD-P of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping near the C-terminus of HGTD-P of human origin |
|---|---|
| Tissue Specificity | epitope mapping near the C-terminus of HGTD-P of human origin |
| Entrez Gene | 26355 |
|---|
Application
Western blot analysis of E2ig5 expression in non-transfected: (A) and mouse E2ig5 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of HGTD-P of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
