Application
Western blot analysis of TRIAD3 expression in mouse kidney tissue extract.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic localization.| Product Name | TRIAD3 Antibody (D-23) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CAHH,E3 ubiquitin ligase TRIAD3,RNF216,Triad domain-containing protein 3,ring finger protein 216 |
| Conjugation | Agarose |
| Modification | Acetylated |
| Modification Notes | TRIAD protein family members typically contain a double RING finger-linked (DRIL) domain, which consists of 2 RING fingers flanked by a conserved cysteine-rich zinc-binding region, allowing them to interact with several other proteins. TRIAD3 (Triad domain-containing protein 3), also known as E3 ubiquitin-protein ligase RNF216 or Zinc finger protein inhibiting NF-kappa-B (ZIN), is a 866 amino acid cytoplasmic protein that acts as a E3 ubiquitin-protein ligase and enhances ubiquination, and therefore degradation, of TLR4 and TLR9. This evidence suggests that TRIAD3 may regulate the duration and intensity of Toll-like receptor signaling. TRIAD3, which is highly expressed in testis and peripheral blood lymphocytes, also inhibits IL-1 and TNF-induced NF˚B activation pathways and promotes RIP and TNF-mediated apoptosis. There are three isoforms of TRIAD3 that exist as a result of alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHCIPWB |
| WB:1:100~1:500 IP:1:20~1:100 IF:1:5~1:20 IHC:1:10~1:50 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | CAHH E3 ubiquitin ligase TRIAD3 RNF216 Triad domain-containing protein 3 ring finger protein 216 |
|---|---|
| Tissue Specificity | recommended for detection of TRIAD3 of mouse and human origin by WB, IP, IF, IHC(P), ELISA |
| Entrez Gene | 54476 |
|---|
Application
Western blot analysis of TRIAD3 expression in mouse kidney tissue extract.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic localization.| Application Notes | WB:1:100~1:500 IP:1:20~1:100 IF:1:5~1:20 IHC:1:10~1:50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
