Application
Western blot analysis of GCK expression in Hep G2 whole cell lysate (A) and mouse liver tissue extract (B).Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic and membrane localization.| Product Name | GCK Antibody (W-24) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of GCK of mouse and human origin by WB, IP, IF, IHC(P), ELISA |
| Modification Notes | Glucokinase (also designated hexokinase IV, HXKIV or GCK) plays a key role in the regulation of glucose-induced insulin secretion. GCK is expressed in pancreatic beta cells where it functions as the major glucose sensor of the body, determining the “set point” for insulin secretion. GCK is also expressed in the liver, where it catalyzes the first committed step in the disposal of glucose. Phosphorylation of glucose by glucokinase appears to be the rate-limiting step for glucose catabolism. A lack of glucokinase activity leads to reduced insulin secretion and hyperglycemia, and has been implicated as a cause for maturity onset diabetes of the youth (MODY). In fact, heterozygous point mutations in the gene encoding GCK have been detected in individuals suffering from MODY. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of GCK of mouse and human origin by WB IP IF IHC(P) ELISA |
|---|---|
| Tissue Specificity | recommended for detection of GCK of mouse and human origin by WB, IP, IF, IHC(P), ELISA |
| Entrez Gene | 2645 |
|---|
Application
Western blot analysis of GCK expression in Hep G2 whole cell lysate (A) and mouse liver tissue extract (B).
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic and membrane localization.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
