Application
Western blot analysis of GPR177 expression in Jurkat whole cell lysate.| Product Name | GPR177 Antibody (S-25) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of GPR177 of mouse and human origin by WB, IF, IHC(P) and ELISA; also reactive with additional species, including canine |
| Modification Notes | G protein-coupled receptors (GPRs), also known as seven transmembrane receptors, heptahelical receptors or 7TM receptors, comprise a superfamily of proteins that play a role in many different stimulus-response pathways. G protein coupled receptors translate extracellular signals into intracellular signals (G protein activation) and they respond to a variety of signaling molecules, such as hormones and neurotransmitters. GPR177 (G protein-coupled receptor 177), also known as EVI, MRP, WLS or C1orf139, is a 541 amino acid multi-pass membrane protein that plays an essential role in Wnt-mediated cell-cell communication. The gene encoding GPR177 maps to human chromosome 1 and is expressed as multiple alternatively spliced isoforms. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIFIHCELISA |
| WB:1:500~1:2000 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of GPR177 of mouse and human origin by WB IF IHC(P) and ELISA also reactive with additional species including canine |
|---|---|
| Tissue Specificity | recommended for detection of GPR177 of mouse and human origin by WB, IF, IHC(P) and ELISA; also reactive with additional species, including canine |
| Entrez Gene | 79971 |
|---|
Application
Western blot analysis of GPR177 expression in Jurkat whole cell lysate.| Application Notes | WB:1:500~1:2000 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
