Application
Western blot analysis of NDUFV1 expression in human heart tissue extract.Application
Western blot analysis of NDUFV1 expression in Hep G2 whole cell lysate.| Product Name | NDUFV1 Antibody (H-23) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of NDUFV1 of mouse, rat and human origin by WB, IP and ELISA |
| Modification Notes | Complex 1 (also known as NADH dehydrogenase) of the electron transport chain (respiratory chain) is an enzymatic complex that catalyzes the transfer of electrons from NADH to ubiquinone. Free energy from the reaction is conserved in the transfer of protons into the inter-membrane space to create an electrochemical proton gradient, a driving force for ATP synthesis. Complex 1 is an extremely complicated, multi-protein, L-shaped complex composed of 45 different subunits located in the mitochondrial inner-membrane. NDUFV1 (NADH dehydrogenase ubiquinone flavoprotein 1), also known as UQOR1 or CI-51kD, is a 464 amino acid core subunit protein of complex 1 that is essential for the electron transport chain. The peripheral membrane protein, NDUFV1, contains a highly conserved NADH-binding site and is localized to the matrix side of the inner membrane. Defects in the gene encoding NDUFV1 can cause complex 1 deficiency in humans, which can to lead to Leigh syndrome (LS), a severe neurological disorder characterized by lesions in the subcortical brain region. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of NDUFV1 of mouse rat and human origin by WB IP and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of NDUFV1 of mouse, rat and human origin by WB, IP and ELISA |
| Entrez Gene | 4723 |
|---|
Application
Western blot analysis of NDUFV1 expression in human heart tissue extract.
Application
Western blot analysis of NDUFV1 expression in Hep G2 whole cell lysate.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
