Application
Western blot analysis of apoH expression in Hep G2 whole cell lysate.| Product Name | apoH Antibody (H-23) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of apoH of human origin by WB, IP and ELISA; also reactive with additional species, including canine |
| Modification Notes | Human apolipoprotein H (apoH, also designated b 2-glycoprotein I, activated protein C binding protein or APC inhibitor) is a five-domain plasma membrane-adhesion protein that is rich in sialic acid linked a to galactose or N-acetylgal-actosamine. ApoH has been implicated in a variety of physiological pathways, including blood coagulation and the immune response. ApoH is a cofactor for the binding of serum auto-antibodies from antiphospholipid syndrome, and is correlated with thrombosis, lupus erythematosus and recurrent fetal loss. In addition, apoH is also implicated in the clearance of apoptotic bodies from the circulation. The apoH gene is located on human chromosome 17q23-24. ApoH is synthesized by hepatocytes and is present in blood associated with plasma lipoproteins. ApoH displays a genetically determined structural polymorphism including three alleles (apoH*1, apoH*2 and apoH*3). ApoH can inhibit the translocation of cholesterol from extracellular pools to macrophages, which reduces the cellular accumulation of cholesterol, suggesting that apoH may play an important role in the prevention of atherosclerosis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of apoH of human origin by WB IP and ELISA also reactive with additional species including canine |
|---|---|
| Tissue Specificity | recommended for detection of apoH of human origin by WB, IP and ELISA; also reactive with additional species, including canine |
| Entrez Gene | 350 |
|---|
Application
Western blot analysis of apoH expression in Hep G2 whole cell lysate.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
