Application
Western blot analysis of PARP-3 expression in non-transfected: (A) and mouse PARP-3 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of PARP-3 expression in non-transfected: (A) and human PARP-3 transfected: (B) 293T whole cell lysates and mouse kidney tissue extract (C).Application
Western blot analysis of PARP-3 expression in Hep G2 whole cell lysate.Application
Western blot analysis of PARP-3 expression in non-transfected: (A) and mouse PARP-3 transfected: (B) 293T whole cell lysates.| Product Name | PARP-3 Antibody (B-22) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | raised against synthetic PARP-3 peptide of human origin |
| Modification Notes | Poly(ADP-ribose) polymerase-3 (PARP-3) is part of the base excision repair (BER) pathway, catalyzing the poly(ADP-ribosyl)ation of nuclear proteins. Poly(ADP-ribosyl)ation, a post-translational modification following DNA damage, appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. PARP-3 is a nuclear, DNA-binding protein, which interacts with PARP-1. PARP-3 is present in actively dividing tissues with highest levels in the kidney, skeletal muscle, liver, heart and spleen. Human PARP-3 maps to chromosome 3p21-3-p21.1, a gene region that undergoes alteration in solid malignant tumors. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of PARP-3 of mouse and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | raised against synthetic PARP-3 peptide of human origin |
|---|---|
| Tissue Specificity | raised against synthetic PARP-3 peptide of human origin |
| Entrez Gene | 10039 |
|---|
Application
Western blot analysis of PARP-3 expression in non-transfected: (A) and mouse PARP-3 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of PARP-3 expression in non-transfected: (A) and human PARP-3 transfected: (B) 293T whole cell lysates and mouse kidney tissue extract (C).
Application
Western blot analysis of PARP-3 expression in Hep G2 whole cell lysate.
Application
Western blot analysis of PARP-3 expression in non-transfected: (A) and mouse PARP-3 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of PARP-3 of mouse and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
