Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human urinary bladder tissue showing cytoplasmic staining of squamous epithelial cells.Application
Intracellular FCM analysis of fixed and permeabilized BJAB cells. Black line histogram represents the isotype control, normal rabbit IgG: .Application
Western blot analysis of Syk expression in BJAB (A), Ramos (B), NAMALWA (C) and RAW 264.7 (D) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed RAW 264.7 cells showing cytoplasmic staining.| Product Name | Syk Antibody (N-19) PE |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the N-terminus of Syk of human origin |
| Modification Notes | Syk (spleen tyrosine kinase) is a 635 amino acid protein that contains one protein kinase domain and two SH2 domains. One of several members of the protein kinase superfamily, Syk functions as a positive effector of B cell antigen receptor (CD79)-stimulated responses, coupling CD79 with the movement of one calcium ion through one of two phospho-regulated pathways. Specifically, calcium ions travel through either a phosphoinositide 3-kinase (PI 3-kinase)-dependent pathway when Syk is not phosphorylated, or through a phospholipase C (PLC) ©-dependent pathway when human Syk is phohsphorylated on Tyr 348 and Tyr 352. Via its ability to influence CD79 activity and to control the movement of calicum through the cell, Syk plays an important role in a variety of cellular responses, including differentiation, phagocytosis, proliferation and B cell development. Syk expression is upregulated in T cell lymphoma, suggesting a possible role for Syk in tumorigenesis. Two isoforms of Syk, designated short and long, exist due to alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCFCELISA |
| recommended for detection of Syk of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the N-terminus of Syk of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the N-terminus of Syk of human origin |
| Entrez Gene | 6850 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human urinary bladder tissue showing cytoplasmic staining of squamous epithelial cells.
Application
Intracellular FCM analysis of fixed and permeabilized BJAB cells. Black line histogram represents the isotype control, normal rabbit IgG: .
Application
Western blot analysis of Syk expression in BJAB (A), Ramos (B), NAMALWA (C) and RAW 264.7 (D) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed RAW 264.7 cells showing cytoplasmic staining.| Application Notes | recommended for detection of Syk of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
